Hematological analysis process 1 (ILV)

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Course numberB3.06651.20.280
Course codeHämaAP1
Curriculum2019
Semester of degree program Semester 2
Mode of delivery Presencecourse
SPPW2,0
ECTS credits2,0
Language of instruction German

Based on the understanding of the physiological and pathophysiological regulatory mechanisms of hematopoiesis, students are able to perform the biomedical analysis process in hematology, i.e.:

  • To assess the central importance of accurate sample identification
  • Assign the relevant measures to the respective bioanalytical and medical question and understand the requirement with regard to the requested analyses and to assess whether the available examination material is suitable for the requested biomedical analyses
  • Assess the preanalytical influence and method-dependent disturbance variables on the respective measured variables and their effects on the respective laboratory test
  • Critically evaluate the hematological analysis methods, in particular manual techniques such as cell counting, staining and cell differentiation, and to justify them based on the question
  • Recognize the basic reaction processes in the analysis process or recognize them in the automated processes
  • Apply suitable evaluation methods or methods of data acquisition based on their knowledge of the measurement process, evaluation, method-specific measured values, measurement results and calculation methods, as well as to correlate them with the functionality of current and new equipment technologies
  • Apply appropriate laboratory analytical procedures and techniques, and perform selected hematological and hemostaseological analyses (coagulation and fibrinolysis analysis, etc.) with the appropriate measurement, detection and assessment procedures and with the help of work instructions using different sample materials
  • Carry out and assess subject-specific quality assurance measures
  • Identify and correctly allocate simple mistakes in the execution of a method and remedy them as far as possible
  • Documentation of examination results
  • Properly carry out the storage and transport of examination materials and reagents
  • Apply laboratory-specific hygiene measures appropriately and according to requirements
  • Technically and biomedically validate the assay results
  • Based on the available laboratory results, students can logically select possible further laboratory analyses (step-by-step diagnostics)
In addition, students are able to independently acquire capillary and venous specimens according to a doctor's order and to further process them for the requested biomedical analysis.

Modules of the 1st semester

  • Hematological analysis process (pre-analytics, analytics, post-analysis including quality assurance, documentation, validation and interpretation of results, transmission, archiving of findings)
  • Extraction, transport, storage and disposal of hematological analysis materials (blood and bone marrow materials)
  • Basics of automated particle counting
  • Manual and automated determination of blood count and differential blood parameters, interpretation, including evaluation of current histograms and scattergrams
  • Spreading techniques and staining protocols, including panoptic stainings
  • Microscopic description and differentiation of physiologically occurring hematopoietic cells in peripheral blood including reactive and erythrocytic changes

Mahlberg R. et al. (2012), Hämatologie: Theorie und Praxis für medizinische Assistenzberufe, Wiley-VCH Verlag;
Hallbach J. (2019), Klinische Chemie und Hämatologie: Biomedizinische Analytik für MTLA und Studium, Thieme;
Haferlach T. et al. (2019), Taschenatlas Hämatologie: Mikroskopische und klinische Diagnostik für die Praxis, Thieme;
Freund M. (2008), Praktikum der mikroskopischen Hämatologie: Begründet von Fritz Heckner, Elsevier;
Moore G. et al. (2016) Haematology, Oxford New York: Oxford University Press;
Fangerau H. et al. (2017), Medizinische Terminologie - ein Kompaktkurs, Lehmanns Media;
Michl M. (2016), Hämatologie - Basics, Elsevier;
Books, journals and websites according to the current state of knowledge in the respective fields.

Lectures, demonstrations, laboratory exercises, discussions