Medical-chemical analysis process 1 - main focus: physicochemical, enzymological and immunochemical and electrochemical analysis methods Group 2 (ILV)

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Course lecturer:

Dr.in med. univ.

 Carina Brenner , BSc

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 Raphael Gassner , BSc

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FH-Prof. Mag.a Dr.in

 Astrid Paulitsch-Fuchs

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 Marion Waldner , BSc

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Course numberB3.06651.20.300
Course codeMC1
Curriculum2019
Semester of degree program Semester 2
Mode of delivery Presencecourse
SPPW2,0
ECTS credits2,0
Language of instruction German

Based on the understanding of the physiological and pathophysiological regulatory mechanisms of the human organism, students are able to perform the biomedical analysis process in medical chemistry, i.e.:

  • Assess the central importance of accurate sample identification;
  • Assign the relevant measures to the respective bioanalytical and medical question and understand the requirement with regard to the requested analyzes and to assess whether the available examination material is suitable for the requested biomedical analyzes
  • Assess the preanalytical influence and method-dependent disturbance variables on the respective measured variables and their effects on the respective laboratory test
  • Critically evaluate the medical-chemical analysis methods, in particular physicochemical, enzymological, immunochemical and electrochemical methods, and to justify them based on the research question
  • Recognize the basic reaction processes in the analysis process or recognize them in the automated processes
  • Apply suitable evaluation methods or methods of measured value acquisition based on their knowledge of the measurement process, evaluation, method-specific measured values, measurement results and calculation methods as well as to correlate them with the functionality of current and new equipment technologies
  • Apply appropriate laboratory analytical procedures and measures, and to carry out selected medical chemical analyzes (focusing on physicochemical, enzymological, immunochemical, electrochemical, CSF and urinary) utilizing the appropriate measurement, detection and assessment methods as well as to carry out work instructions whilst using different sample materials
  • Conduct and assess subject-specific quality assurance measures
  • Identify and correctly allocate simple mistakes in the execution of a method and remedy them as far as possible
  • Documentation of the results of the examination
  • Properly carry out the storage of examination materials and reagents
  • Apply laboratory-specific hygiene measures appropriately and according to requirements
  • Technically and biomedically validate the test results and, based on the present laboratory results, logically select possible further laboratory analyzes
In addition, students are able to independently acquire capillary and venous specimens according to a doctor's order and to further process them for the requested biomedical analysis.

Modules of the 1st semester

  • Physicochemical analysis methods: methods for the separation of mixtures of substances (electrophoresis, chromatography), absorption and flame photometry including pre- and post-analysis exemplary for the laboratory analysis of carbohydrate metabolism (eg glucose, lactate, HbA1c), plasma proteins, electrolytes (Na+, K+), the metabolic end products (creatinine, urea, uric acid, bilirubin) and chemical urine diagnostics
  • Microscopic analysis of urinary and cerebellar elements (laboratory exercises on the microscope)
  • Enzymological analysis methods: Methods of enzymatic analysis (enzyme kinetics, enzyme activity) including pre- and postanalytics exemplary for laboratory analysis of diagnostically important enzymes and organ-specific enzyme diagnostics (for example ALAT, ASAT, gGT, LDH, CHE, AP, amylase, CK)
  • Electrochemical analysis methods: potentiometry, coulometry, amperometry incl. pre- and post-analysis exemplarily for the laboratory analysis of the acid-base household / the blood gases and the electrolytes
  • Immunochemical and radioactive analysis methods: immunoassays / radioimmunoassays, turbidimetry, nephelometry, complement fixation, immunodiffusion, immunofixation including pre- and post-analysis exemplarily for the laboratory analysis of tumor markers and in the context of inflammatory diagnostics and hormone diagnostics.
  • Quality assurance (internal, external QC), selection of methods and equipment, laboratory organization, laboratory-specific legislation and safety regulations in the medical laboratory
  • Laboratory exercises on physicochemical, enzymological, immunochemical and electrochemical analytical procedures, including documentation, technical and biomedical validation of results (including workplace preparation and follow-up)

Hallbach J. (2019), Klinische Chemie und Hämatologie: Biomedizinische Analytik für MTLA und Studium, Thieme;
Thomas L. (2012), Labor und Diagnose, TH-Books;
Hallwachs-Baumann G. (2011), Labormedizin, Springer;
Renz H. (2003), Integrative Klinische Laboratoriumsmedizin, De Gruyter;
Böhm B. O. (2018), Klinikleitfaden Labordiagnostik, Urban &Fischer;
Dörner K. (2019), Klinische Chemie und Hämatologie, Thieme;
Books, journals and websites according to the current state of knowledge in the respective fields.

Lectures, laboratory exercises, demonstrations, discussions