Analytical methods in genetic engineering and molecular biology (ILV)

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Course numberB3.06651.40.490
Course codegentechAV
Curriculum2019
Semester of degree program Semester 4
Mode of delivery Presencecourse
SPPW2,0
ECTS credits2,0
Language of instruction German

The students develop methodological skills in molecular biology, genetic engineering, biochemistry, immunology and cell biology which are required for biomedical analysis or R & D projects, i.e. they can understand the respective analysis methods, they can independently plan, carry out and evaluate them and judge the results of their analysis. Students are able to verify the suitability of the sample material for the requested molecular biological analysis, to obtaining missing relevant information or to request the submission of new samples. The students can carry out subject-specific measures in the context of the analytical process. They are able to recognize method-specific technical and biological confounding factors in the context of quality assurance, validate the analysis results and document, transmit and archive them with the respective information technologies. They can logically select advanced laboratory analytical methods and assess the importance of analytical results for clinical diagnostics and research and development projects. They can relate and implement the analytical process with an understanding of current and new device technologies. Students are able to store study materials and reagents properly. In addition, students can use information technologies for subject-specific data research, adapt new examination procedures based on manuals or scientific literature and implement or carry out new tasks with the help of literature. They know about the need to track current developments and integrate them into their work, know research-related aspects and can apply the acquired knowledge in research, science, industry or in veterinary medicine.

Modules of the 1st, 2nd and 3rd semester

  • Preanalytics in molecular biology / genetic engineering
  • Planning, implementation and evaluation of molecular biological and genetic engineering analysis methods (restriction enzyme digestion, blotting, cloning) including troubleshooting and the assessment of results
  • Special genetic engineering applications / molecular biological methods in research & development (e.g. SNP, transgenic mouse models)
  • Bioinformatic aspects in molecular biology, using genetic databases (e.g., NCBI, Ensembl)
  • Aspects of quality assurance in genetic engineering, biological safety and hygiene, genetic engineering law and subject-specific guidelines, documentation
  • Importance of molecular biological and genetic methods for the accurate and rapid diagnosis of diseases of molecular medical relevance, including monitoring and research and development

Mülhardt C. (2008), Der Experimentator Molekularbiologie / Genomics, Spektrum;
Schartl M. et al. (2009), Biochemie und Molekularbiologie des Menschen, Thieme;
Alberts B. et al. (2017), Molekularbiologie der Zelle, Wiley-VCH Verlag;
Jacobi B. & Partovi S. (2010), Molekulare Zellbiologie - Basics, Elsevier;
Reinard T. (2018), Molekularbiologische Methoden 2.0, UTB;
Jansohn M. & Rothhämel S. (2012), Gentechnische Methoden: Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor, Spektrum;
Books, journals and websites according to the current state of knowledge in the respective fields.

Lectures, laboratory exercises, reflections, discussions